Mispa Lume is a highly compact molecular diagnostics system to simultaneously perform 16 samples at faster TAT for real time detection of target genes using RT-LAMP reaction.
Description
Mispa Lume is a very small molecular diagnostics machine that can run 16 samples simultaneously at a quicker TAT for real-time target gene identification utilizing RT-LAMP reaction.
What is LAMP Technology:
LAMP is the short form for Loop-mediated isothermal amplification, a gene amplification assay for detecting the target gene. LAMP is faster than traditional PCR at analyzing genetic material. Reverse-transcription LAMP (RT-LAMP) amplifies RNA gene targets and has been used to find the COVID-19 virus. RT-LAMP with crude RNA samples can be used for quick testing at the point of care because it can use patient samples directly. It is crucial to select a diagnostic technique that is simpler and quicker than RT-PCR, depending on the circumstance. Both RT-LAMP and LAMP use four to 6 primers that recognize the target DNA. A strand-displacing DNA polymerase starts the process, and 2 of the primers form loop structures that make it easier for the next amplification round.
Specialties of an RT-LAMP Analyzer
RT-LAMP requires a constant temperature range from 60-72°C. InstaLume RT-LAMP analyzer is capable of maintaining a constant temperature and real-time fluorescence detection that enables real-time gene amplification monitoring. The InstaLume analyzer can run 1 to 16 samples at a time. The results can be obtained regarding Ct (Cycle threshold) values. One of the advantages of this technology is that the consumables require only 2-8*C,