Taq DNA polymerase reagent / buffer solution / dNTP solution / for PCR
TEMPase Master Mix

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Taq DNA polymerase reagent Taq DNA polymerase reagent - TEMPase Master Mix


  • Type:

    buffer solution, dNTP solution, Taq DNA polymerase

  • Applications:

    for PCR

  • Other characteristics:

    hot start


TEMPase Hot Start Master Mix
TEMPase Hot Start Master Mix is a ready to use mix that offers easy reaction assembly at room temperature prior to hot start PCR. Just add your template and primers to successfully carry out PCR.
TEMPase Hot Start Master Mix is the popular alternative to TEMPase Hot Start DNA Polymerase. It offers the same excellent performance and increased reproducibility.


Minimal optimisation
Convenient reaction set up at room temperature
Time-saving premixed solution
Increased sensitivity, specificity and product yield
Consistent test results
Lower risk of contamination


Ampliqon TEMPase Hot Start DNA Polymerase 2x Master Mix is a ready to use master mix composed of TEMPase Hot Start DNA Polymerase, dNTPs, MgCl2 and either TEMPase Buffer C (a balanced KCI/(NH4)2 SO4 Tris buffer system) or TEMPase Buffer A (a (NH4)2 SO4 tris buffer system).

The TEMPase buffer system covers all needs for PCR applications and allow the user to choose the optimal buffer for specific PCR set-ups and applications.


For most standard applications Master Mix A based on Ammonium Buffer is the best option. It promotes robust amplification, high yield and high specificity. In certain cases, Master Mix C is the best option.

If you want to visualise on agarose gels, we suggest that you choose Master Mix BLUE A or master Mix BLUE C.