Cancer assay kit

for NRAS mutationscellfor PCR

Applicable instrument: Stratagene Mx3000P Product description Adopting the ARMS-PCR method, this kit can be used to detect 9 hot-spot mutations in codons 12 and 13 of exon 2 and codon 61 of exon 3 of the NRAS gene. This kit is used for qualitative assay of NRAS mutation(s) in patients with non-small cell lung cancer (NSCLC) and colorectal cancer. The product contains our proprietary stabilizer with which high specificity can be guaranteed and false positives can be avoided under the situation when the sensitivity reaches 1%. The assay result can be used as the reference for selection of population who may receive iressa (gefitinib), tarceva (erlotinib), and conmana (icotinib) among NSCLC patients, and population who may receive erbitux (cetuximab) and vectibix (panitumumab) among patients with colorectal cancer. The product can be used in in vitro tests only. The assay result can only be used for clinical reference and shall not be used as the only basis for diagnosis and treatment. Product Features High selectivity——Detect as low as 1% of mutations in 5 ng] DNA from multiple types of samples High sensitivity——Only a few hundred copies are needed to detect the target gene High specificity The amplification curve is flattening when wild type samples are tested Easy to operate—— Pre-assembled to facilitate standard operation in the lab Convenient and fast——Suitable for various PCR instruments, test completed in 90 minutes Stable and reliable——Closed tube reaction, IR-IC dual QC to avoid false negatives and positives Convenient read-out——No non-specific amplification, read the result directly, no need to manually calculate the =CT value