DNA polymerase reagent PR100-HF series

for PCR

For applications requiring highly accurate amplification, Accuris offers a range of high fidelity PCR options. Our proofreading enzymes are modified for better sensitivity and higher activity, and this ensures more reproducible performance with a wide range of targets. Up to 100X higher fidelity than Taq polymerase. Crude DNA sample will not inhibit amplification. Ideal for cloning, mutagenesis, sequencing and microarrays. Options for blunt end or A-overhang products. Optimized buffers include PCR enhancers. Accuris High Fidelty Polymerase (2 tube formulation) Leaves a blunt end for blunt end vector cloning 50x fidelity of native Taq Ideal for complex targets up to 10kb For applications requiring highly accurate amplification, and full control of enzyme concentration, choose our High Fidelity DNA polymerase kit. Our polymerase enzyme has been genetically modified for better solubility and higher activity across a broad range of ionic conditions, and will amplify a wide range of targets, including those that are GC or AT rich as well as crude DNA samples. A 3′-5′ proofreading exonuclease activity and an error rate of 4.55×10-7 makes Accuris High Fidelity DNA Polymerase the perfect partner for cloning applications. The included 5X buffer contains an optimized concentration of dNTPs and enhancers for compatibility with a variety of amplicons up to 10kb in length. Accuris High Fidelity Master Mix Leaves a blunt end for blunt end vector cloning 100x fidelity of native Taq Ideal for shorter, less complex targets