Lipase reagent kit LIP-20 series

enzymesolutiondye

For the quantitative determination of lipase in serum or plasma. For in vitro diagnostic use only. METHOD HISTORY This method is based on the cleavage of a specific chromogenic lipase substrate 1,2-O-dilauryl-rac-glycero-3-glutaric acid-(6-methylresorufin) ester emulsified with bile acids. The pancreatic enzyme activity is determined specifically by the combination of bile acid and colipase used in this assay. Virtually no lipase activity is detected in the absence of colipase. Colipase only activates pancreatic lipase, but not other lipolytic enzymes found in serum. The high amount of cholates ensure that the esterases present in the serum do not react with the chromogenic substrate due to the highly negative surface charge. PRINCIPLE At alkaline pH the lipase dye substrate 1,2-O-dilauryl-rac -glycero - 3 - glutaric acid - ( 6 -methyl - resorufin) esther is cleaved by the catalytic action of pancreatic lipase to form 1,2-O-dilauryl-rac-glycerol and unstable compound glutaric acid-(6-methyl-resorufin) – esther. In that alkaline solution,this compound degrades to glutaric acid and methylresorufin.The color intensity of the red dye formed is directly proportional to the lipase activity. This activity can be measured at 578 nm. PRECAUTIONS 1. The reagents contain Sodium azide at 0.09%. 2. Handle with care. The disposal of the residues has to be made according to legal local regulations.. Concentrations of Hemoglobin higher than 400 mg/dL and of Bilirubin higher than 60 mg/dL can interfere with the assay. To perform this test, the use of disposable labware is highly recommended.