-Hot Start PCR.
Titan HotTaq DNA Polymerase (Fig. 1) is a modified Titan Taq DNA Polymerase. At ambient temperatures it is inactive, having no polymerase activity. Titan HotTaq DNA Polymerase is activated by a 15 minute incubation at 95-97°C, preventing the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5’ to 3’ polymerisation-dependent exonuclease replacement activity but lacks 3’ to 5’ exonuclease activity. The enzyme has “extendase activity” allowing TA cloning.
Comments: This product is supplied for research use only.