LyoFACT™ All in one PCR Pre-Mix includes primers on the kit and it can conveniently be used by adding template RNA & RNase free water only. It reduces and minimizes the possibility of contamination & varation during PCR amplification.
■ Feature
• Source : Thermus aquaticus
• 5' → 3' exonuclease activity : Yes
• 3' → 5' exonuclease activity (Fidelity) : No
• IT102A-096 [M13(-20)F,M13(-20)R] IT102B-096 [M13(-47)F,M13(-48)R] / IT103-096 [E.coli MLST Primer Set]
• 12-13 bp error / 106 bp
• A-tailing : Yes
• Pre-Mix with primers on the package
• High solubility
• Minimization of temperature restriction
■ Application
• Routine PCR • PCR screening • TA cloning • Colony PCR