Veterinary detection kit EB4301PO

animalHLA-B27for antibodies

Indircet ELISA kit for detectiont of antibody against Bruella abortus in bovine serum, plasma, or milk Indications • Diagnosis of bovine brucellosis in lab • Screening for test and slaughter government policy • Massive screening for bovine import & export market Spécial Features • Fully meets the requirement of EU Council Directive and the OIE Manual • Standardized by OIE standard sera (B.abortus 1119-3) • No cross reaction with yersinia enterocolitica Quick Procedure 1. Prepare LPS coated test plate 2. Dilute test samples 1:49 with sample diluent (Do not dilute contrais and Milk) 4. Incubate the plate for 60 minutes at 37°C 5. Wash plate 5 times 6. Add 100 pl of diluted enzyme conjugate to wells 7. Incubate plate for 30minutes at 37°C 8. Wash plate 5 times 9. Add 100 pl of mixed substrate (Ready to use) to each well and incubate for 15 minutes at room temperature 10. Add 100 plofstopping solution 11. Measure the optical density (OD) at 450nm with reference wavelength at 620nm 12. %Pvalue=[ODsampie/meanODpc]x100