Oxidative stress assay kit KB03011
superoxide dismutaseenzymaticcolorimetric

oxidative stress assay kit
oxidative stress assay kit
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Characteristics

Applications
for oxidative stress
Tested parameter
superoxide dismutase
Analysis mode
enzymatic, colorimetric

Description

KB03011 | Superoxide dismutase, SOD Activity Assay Kit SOD Activity Assay Kit (Colorimetric) is a sensitive kit using WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method. Description Superoxide dismutases (SODs) are metallo enzymes that catalyse the dismutation of the superoxide anion to molecular oxygen and hydrogen peroxide. Thus, it forms a crucial part of the cellular antioxidant defense mechanism. Bioquochem’s SOD Activity Assay Kit utilizes a tetrazolium salt for detection of superoxide radicals. Particularly, xantine oxidase and hypoxantine are the enzymes that generate these radicals (see scheme). Excessive reactive oxygen species, especially superoxide anion (O2•−), play important roles in the pathogenesis of many cardiovascular diseases, including hypertension and atherosclerosis. Superoxide dismutases (SODs) are the major antioxidant defense systems against O2•−. There are three isoforms of SOD in mammals: the cytoplasmic Cu/ ZnSOD (SOD1), the mitochondrial MnSOD (SOD2), and the extracellular Cu/ZnSOD (SOD3). These enzymes require catalytic metal (Cu or Mn) for their activation.

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.