Oxidative stress assay kit KB03011
superoxide dismutaseenzymaticcolorimetric

oxidative stress assay kit
oxidative stress assay kit
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Characteristics

Applications
for oxidative stress
Tested parameter
superoxide dismutase
Analysis mode
enzymatic, colorimetric

Description

Superoxide dismutases (SODs) are metalloenzymes that catalyze the dismutation of the superoxide anion to molecular oxygen and hydrogen peroxide. SOD Activity Assay Kit bases on the reduction of WST-1, which produces a water-soluble coloured formazan dye. The rate of the reduction with a superoxide anion linearly relates to the xanthine oxidase (XO) activity. By contrast, SOD inhibits this reaction. Therefore, the inhibition activity of SOD can be determined by a colorimetric method. Specifically, one unit of SOD corresponds to the amount of enzyme needed to exhibit 50% dismutation of the superoxide radical. The conversion of xanthine and O2 to uric acid and H2O2 by XOD generates superoxide radicals. As a result, the superoxide anions reduce a tetrazolium salt (WST-1) to a colored formazan product (WST-1 formazan) that absorbs light. SOD scavenges superoxide anions, thereby reducing the rate of formazan dye formation.

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