Solution reagent Accutase®

enzymefor cell culturefor cell counting

Product overview
Accutase® is a ready-to-use cell detachment solution composed of proteolytic and collagenolytic enzymes. It replaces trypsin/EDTA for many passaging workflows and can be used on suspended cells to reduce clumping before counting. The product is supplied frozen and sterile-filtered for cell culture applications.

Effective on

• Fibroblasts
• Keratinocytes
• Vascular endothelial cells
• Hepatocytes
• Vascular smooth muscle cells
• Bone marrow stem cells
• Adherent CHO and BHK cells
• 293 cells
• L929 cells
• 3T3 cells
• Vero
• HeLa

Applications
Cell culture passaging, detachment of adherent cells, reduction of cell aggregation for counting and downstream processing.

Main advantages of Accutase

• Reduced proteolytic damage compared with trypsin in many cell types
• Improved post-detachment cell viability
• Lower risk of introducing adventitious agents due to sterile filtration and controlled formulation
• Significant reduction of mammalian- or bacterial-derived proteins that can interfere with serum/protein-free workflows
• Increased efficiency of cell seeding and more uniform single-cell suspensions
• Preserves cell morphology and supports proliferation after reseeding
• Suitable for cells cultured in serum/protein-free conditions

Additional information
(Accutase is a registered trademark of Innovative Cell Technologies, Inc.) Other packaging options available on request.

Technical specifications

• Product code: L0950
• State: Frozen
• Shipping conditions: Gel pack, dry ice, refrigerated transport
• Shelf life: 24 months
• Storage: -18°C to -40°C
• Sterility: Sterile filtered
• Intended use: Cell detachment for adherent and suspension cultures