Buffer solution reagent L0180 series

HEPESfor researchfor biochemistry

Product overview
HEPES is a zwitterionic organic buffering agent commonly used to stabilize pH in cell culture media and biochemical assays. At physiological pH (7.2–7.4) and 37°C, HEPES provides greater buffering capacity than sodium bicarbonate.

Applications

• Cell culture media supplementation
• Biochemical and cellular assays
• Laboratory workflows requiring stable pH at 37°C

Recommended concentration / Important note
Typical addition: 10–25 mM. Higher concentrations may cause cytotoxicity and should be validated for each cell line or assay.

Available volumes

• 100 ml
• 500 ml

Product details
Product code: L0180
State: Liquid
Shipping conditions: Room temperature or controlled temperature
Shelf life: 36 months
Storage: +2/+8°C
Sterility: Sterile filtered

Technical specifications

• Concentration (product): 1 M
• Buffer type: Zwitterionic organic buffer
• Effective buffering pH: 7.2–7.4 at 37°C
• Recommended working concentration: 10–25 mM
• Format: Liquid, sterile filtered
• Shelf life: 36 months
• Storage temperature: +2/+8°C
• Catalogue number / SKU: L0180