Ion mobility spectrometer timsTOF HT

masstime-of-flightfor cell culture

Powered by the 4th generation TIMS-XR and advanced digitizer technology for high dynamic range and analytical depth in quantitative cell and tissue proteomics. High-throughput 4D-Proteomics™ High dynamic range and quantitation accuracy Speed PASEF® technology on the timsTOF HT achieves 150 Hz sequencing speed without affecting resolution or sensitivity. Robustness The unique instrument design enables robust performance and routine operation over thousands of samples without requiring cleaning. Sensitivity High charge capacity of the 4th generation TIMS-XR for increased analytical depth. Selectivity Adding ion mobility separation to the advanced quadrupole and Time-of-Flight capacities. TIMS based ion mobility separation and CCS-enabled analysis The new timsTOF HT mass spectrometer is equipped with the 4th generation high capacity TIMS-XR analyzer and advanced digitizer technology (ADT). It offers higher dynamic range for unmatched analytical depth and quantitation in high-throughput proteomics experiments. The timsTOF HT is fully CCS-enabled and supports all PASEF® acquisition modes including PASEF®, dia-PASEF® and prm-PASEF® achieving flexibility in 4D-Proteomics™. Simply uncompromised proteomic depth at scale. Enabling rapid tissue proteome analysis with dia-PASEF® Rapid cardiac tissue proteomics. Different amounts of a murine heart proteome digest were analyzed in triplicates with short liquid chromatography gradients and dia-PASEF®. At the highest sample load, we identified in total 4700 protein groups spanning more than five orders of magnitude in relative abundance.