Accurate Counting of Mammalian Cells
As accurate as FACS counting
Are you looking for an automated cell counter at the pinnacle of accuracy? Many labs have turned to the ADAM MC as a solution that yields highly repeatable results, as with a flow cytometer, but at a fraction of the cost. Its technology is simple; it combines a precision microscope with an integrated fluorescence detection optical system and onboard analysis software. With disposable chip-based kits that use propidium iodide to stain the nucleus of target cells, the ADAM never worries about cell size, shape, or aggregation. Instead it uses fluorescence to specifically image the stained nuclei, and averages 22 separate reads for each sample totaling a 3 µl read volume. These improvements specifically address variation attributable to low sample reads (some competitor models only perform one read), low sampling volumes (most models read 1 µl or less), and inaccurate resolution of cells (often found when cells clump together). With R2 values over 0.99, the ADAM MC can directly replace manual cell counting using the propidium iodide technique. The results are amazing, and it’s possible to get CVs under 10% with most cell lines. The ADAM MC can work with a dizzying range of suspension cell types, as only nuclei are needed for detection, and it uses a very clever “detergent wash” technique to provide precise cell viability values. But don’t take our word for it—we invite you to request a demo and find out for yourself.