Solution reagent kit MicroStacker™

enzymeantibodybuffer solution

MicroStacker™ Mouse-on-Mouse HRP-Polymer Detection Kit PN: RS4001 Components: MicroStacker™ Anti-Mouse HRP-Polymer 20*DAB Chromogen DAB Substrate Buffer Application: for Research Use Only (RUO) Type: Ready-to-Use (RTU) The MicroStacker™ technology allows well-controlled layered stacking of F(ab’) fragments of IgG secondary antibody and peroxidase enzymes on a micro-polymer scaffold, which results in a compact polymeric structure with superior sensitivity compared to other conventional HRP polymers with bulky dextran backbones. This system avoids the use of streptavidin and biotin, and therefore eliminates non-specific staining as a result of endogenous biotin. Herein, the Mouse-on-Mouse Polymer comprises of specially engineered anti-Mouse secondary antibody to eliminate the high backgrounds caused by endogenous mouse antibodies in mouse tissues. Staining Procedure： Primary antibody incubation: RT, 30-60min Secondary antibody incubation: RT, 15min DAB incubation：5min Experimental group: MicroStacker™ Mouse-on-Mouse Detection polymers in comparison: International Famous Brand