Medium reagent

penicillingrowth factorfor cell culture

Peripheral Blood Lymphocyte Medium is specially intended for the cultivation of human peripheral blood lymphocytes, for karyotype analysis. With optimized medium composition, strict quality control and aseptic production process, Celnovte Bio-tech can ensure that lymphocytes are large in size and have multiple metaphases with thick chromosomes and good morphology and clear under the microscope. Product Details Medium Compositions: Basal Medium RPMI1640 High quality fetal bovine serum Optimized concentration of PHA Penicillin & Streptomycin Special packaging tube, sterile, no heat source etc.(for example cell growth factor) Inspection Principle Phytohemagglutinin (PHA) is a stimulant of lymphocyte mitosis and makes lymphocytes begin to mitosis under its action. Lymphocytes are cultured in a medium containing PHA, and a rich population of mitotic cells can be obtained in vitro. The desired karyotype can be observed as appropriate amount of colchicine (mitotic inhibitor) stops the cell mitosis. Advantages: a)Optimization of Culture Medium compositions, excellent cell growth status and high conversion rate (> 80%) b)Excellent product stability, small batch-to-batch differences, shelf life for 8 months c)100,000 class clean production workshops with highly controlled environment,strict quality control. d)Innovative packaging design adding samples without opening the lid, simplify operation and reduce cross-contamination e)Aseptic liquid small packaging to avoid the probability of contamination in large packaging, ready-to-use reagents