Enzyme reagent kit Celsis AMPiScreen®

solutionfor microbial contaminationadenosine triphosphate

Celsis AMPiScreen® reagent technology, in combination with our Celsis®rapid microbial detection instruments, can confirm the presence of ultra-low levels of microbiological contamination up to 50% faster than traditional adenosine triphosphate (ATP) bioluminescence. The inherent subjectivity of manual enumeration or visual confirmation of growth is replaced by a data-derived, confident "Yes/No" answer, resulting in speedier decisions to release or hold products. ATP bioluminescence, a gold standard rapid detection method, provides an effective approach to confidently detect the presence of microbial contamination in a sample. However, it is dependent upon microbial growth to achieve sufficient levels of ATP for detection. Celsis AMPiScreen® reagents utilize a method of amplifying ATP that is not constrained by the finite amount of metabolic ATP available in the standard bioluminescence assay. The amplification of ATP beyond that which is inherent in the test sample is accomplished through a proprietary reagent, Celsis LuminAMP™, and an additional instrument-controlled amplification period to optimize the detection of microorganisms. Based upon a proven detection method, Celsis AMPiScreen® products amplify low levels of contaminant ATP present in a test sample that are then easily detected by bioluminescence within Celsis® instruments. This naturally occurring reaction, which also provides the characteristic glow of fireflies, occurs when the luciferase enzyme converts luciferin into oxyluciferin to produce bioluminescence. This reaction requires the presence of ATP to occur.