Polymerase chain reaction (PCR) is the preferred laboratory test given its accuracy and sensitivity which suggested by WHO. Confirmation of PCR test for monkeypox virus infection is based on nucleic acid amplification testing (NAAT), using real-time or conventional polymerase chain reaction (PCR) like monkeypox detection kit is preferred for detection of unique sequences of viral DNA and can be used alone.
Monkeypox Test Kit Features
Reliable: Based on WHO recommended diagnosis method: real-time polymerase chain reaction (PCR)
High Specificity: Highly conservative region of Monkeypox virus gene coding region
High Accuracy: Involved endogenous internal to monitor the whole NAAT procedure from sampling to PCR result
Universal: Compatible with a mainstream real-time fluorescent PCR system
Test Principle
Real-time fluorescent PCR
Specimen type
Rashes, scabs, blister fluid, pustular fluid, or whole blood specimens
Fluorescence channel
FAM: Monkeypox conservative region, VIC: endogenous internal control
LOD
200 copies/mL
Storage and shelf life
-20±5℃, 12 months
Applicable Instruments
ABI 7500, AGS4800, AGS8830
Certificate
CE
① Specimen collection: take the rash cover or scab, put it into a specimen collection tube, then seal the tube and send it for inspection.
② Specimen processing and nucleic acid extraction: using nucleic acid extraction kit and instrument to obtain purified MPOX DNA.
③ PCR amplification: PCR reagent preparation and using extracted MPOX nucleic acid as PCR template
④ Result analysis