Autoimmune disease test kit EIA3590
IgGIgMserum

autoimmune disease test kit
autoimmune disease test kit
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Characteristics

Applications
for autoimmune diseases
Tested parameter
IgG, IgM
Sample type
serum, plasma
Analysis mode
ELISA
Result display time

5 min, 15 min, 30 min

Sample volume

0.01 ml
(0.00034 US fl oz)

Description

Anti-beta-2-Glycoprotein I IgG/IgM is an ELISA test system for the quantitative measurement of IgG and IgM class autoantibodies against beta-2-Glycoprotein I in human serum or plasma. Highly purified beta-2-Glycoprotein I is bound to microwells. The determination is based on an indirect enzyme linked immune reaction with the following steps: Specific antibodies in the patient sample bind to the antigen coated on the surface of the reaction wells. After incubation, a washing step removes unbound and unspecifically bound serum or plasma components. Subsequently added enzyme conjugate binds to the immobilized antibody-antigen-complexes. After incubation, a second washing step removes unbound enzyme conjugate. After addition of substrate solution the bound enzyme conjugate hydrolyses the substrate forming a blue coloured product. Addition of an acid stops the reaction generating a yellow end-product. The intensity of the yellow color correlates with the concentration of the antibody-antigen-complex and can be measured photometrically at 450 nm. Storage Conditions 2° C - 8° C FDA No CE Y # of Tests 96 wells Range 0 – 100 U/mL (IgG and IgM)

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