Research test kit EIA5298R

for cardiovascular diseasesserumplasma

The DRG Aldosterone ELISA is a manual enzyme immunoassay for the quantitative measurement of aldosterone in human serum or plasma (EDTA, Li-heparin- or citrate plasma) or urine based on the principle of competitive binding. This product is for Research Use Only. International customers contact your representative to receive information about the CE marked version. The microtiter wells are coated with a monoclonal antibody (mouse) directed towards a unique antigenic site of the aldosterone molecule. During the first incubation, the aldosterone in the added enzyme conjugate, which is aldosterone-horseradish peroxidase conjugate for binding to the coated antibody. After a washing step to remove all unbound substances, the solid phase is incubated with the substrate solution. The colorimetric reaction is stopped by addition of stop solution, and optical density (OD) of the resulting yellow product is measured. The intensity of color is inversely proportional to the concentration of the analyte in the sample. A standard curve is constructed by plotting OD values against concentrations of standards, and concentrations of unknown samples are determined using this standard curve.