Thyroid disease test kit EIA4110

anti-thyroglobulinclinicalserum

The Quantitative Determination of Thyroglobulin (Tg) Autoantibodies in Human Serum or Plasma by a Microplate Enzyme Immunoassay, Colorimetric. Measurements of Tg autoantibodies may aid in the diagnosis of certain thyroid diseases such as Hashimoto’s and Grave’s as well as nontoxic goiter. Antibodies to thyroglobulin have been shown to be characteristically present from patients with thyroiditis and primary thyrotoxicosis.1,2 This has lead to the clinical measurement becoming a valuable tool in the diagnosis of thyroid dysfunction. Passive Hemaglutination (PHA) methods have been employed in the past for measurements of antibodies to Tg. PHA tests do not have the sensitivity of enzyme immunoassay and are limited by subjective interpretation. This procedure, with the enhanced sensitivity of EIA, permits the detectability of subclinical levels of antibodies to Tg. In addition, the results are quantitated by a spectrophotometer, which eliminates subjective interpretation. DRG’s microplate enzyme immunoassay methodology provides the technician with optimum sensitivity while requiring few technical manipulations. In this method, serum reference, diluted patient specimen, or control is first added to a microplate well. Biotinylated thyroglobulin (Tg) is added, and then the reactants are mixed. Reaction results between the autoantibodies to Tg and the biotinylated Tg to form an immune complex, which is deposited to the surface of streptavidin coated wells through the high affinity reaction of biotin and streptavidin.