Research assay kit BEK-2233

cellbiologicalELISA

The Eagle Bioscience’s Human LR3 IGF-I ELISA Assay Kit is intended for the quantitative determination of LR3IGF-I in cell culture supernatants. The Eagle Bioscience’s Human LR3 IGF-I ELISA Assay Kit is for research use only Sizes: 1 Plate Kit (12 x 8 wells), 2 Plate Kit (24 x 8 wells) Intended Use The Eagle Bioscience’s Human LR3 IGF-I ELISA Kit has been developed and validated to quantify LR3IGF-I protein in cell culture medium. It is primary use is to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. For research use only and not to be used for diagnostic procedures. Assay Background Human LR3 insulin-like Growth Factor-I (LR3IGF-I) is an 83 amino acid analogue of human IGF-I comprising the complete IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus. Human LR3IGF-I is more potent than IGF-I in vitro and in vivo. This increased potency is due to reduced binding of LR3IGF-I to most of the IGF binding proteins that modify the biological actions of IGF-I. Human LR3IGF-I binds to the type I IGF receptor with similar affinity to wild type IGF-I. LR3IGF-I was developed by GroPep Bioreagents specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin.