Solution reagent Brij® 35

for researchfor protein extractionprotein

Brij® 35 is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution. Proteomic Grade Detergent Solutions Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds. The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and the carbonyl groups will form Schiff’s bases with the proteins that interfere with its structure. G-Biosciences’ Proteomic Grade Detergent Solutions contain reduced peroxides and carbonyl compounds. In addition, the detergents have less than 50µS conductivity. These detergents are offered as sterile, 10% aqueous solutions, sealed under inert gas, and are suitable for protein applications. These non-ionic detergents are suitable for isolating membrane-protein complexes. Features Molecular Weight: 627 Absorbance (225nm): 0.07 (1% w/v) Critical Micelle Concentration (CMC): 90µM Aggregation Number: 24 to 40 Cloud Point: > 100º C Average Micellar Weight: 48,000 Molecular Formula: C₁₀H₂₆O (OCHâ‚‚CHâ‚‚)₁₀ Applications Non-ionic detergent available as a 10% proteomic grade solution or a research grade solid For protein extraction, permeabilization of cells, and preparation of yeast spheroplasts Ultra low concentrations of contaminating peroxides, aldehydes, salts and carbonyl compounds