Taq DNA polymerase reagent 786-44 series
enzymeDNA polymerasefor scientific research

Taq DNA polymerase reagent
Taq DNA polymerase reagent
Add to favorites
Compare this product
 

Characteristics

Type
enzyme, DNA polymerase, Taq DNA polymerase
Applications
for scientific research
Tested parameter
protein, glycerol

Description

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile, Thermus aquaticus. The molecular weight of the recombinant protein is 94kD.. The Taq polymerase is able to amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C. The error rate of this Taq polymerase is ~2.2x10-5 nucleotide-1 cycle-1. Taq DNA polymerase catalyzes the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product SOURCE A recombinant protein expressed in E. coli that carries the pol gene for Thermus aquaticus. STORAGE BUFFER 20mM Tris.HCl (pH8.0), 100mM KCl, 3mM MgCl2, 1mMDTT, 0.1% Nonidet® P-40, 0.1% Tween® 20, 0.2mg/ml BSA, 50% (v/v) glycerol. 10X PCR BUFFER 100mM Tris-HCL pH8.8, 500mM KCl, 16mM MgCl2, 1% TritonX-100 UNIT DEFINITION One unit (U) of Taq polymerase is defined as the amount of enzyme needed to catalyze the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70°C using herring sperm DNA as a substrate. Our Taq DNA Polymerase is offered in four convenient sizes and supplied with 10X PCR buffer (Mg2+ plus). Taq Polymerase 2X Mastermix is a ready-to-use mixture of our high quality Taq DNA polymerase, deoxynucleotides and reaction buffer at a 2X concentration. Features 5’→3’ polymerase activity No detectable 3’→5’ proofreading exonuclease activity Low 5’→3’ exonuclease activity Error rate ~2.2x10-5 nucleotide-1 cycle-1 Available as a 2X Mastermix

Catalogs

No catalogs are available for this product.

See all of G-BIOSCIENCES‘s catalogs
*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.