Reagent kit for the capture of histidine-tagged ligands in Biacore systems, providing an alternative to capture on nickel-chelated nitrilotriacetic (NTA) groups.
Overview
Easy: Minimum assay development with no need to identify reagents or verify assay conditions.
Convenient: Optimized protocol and all required solutions for the efficient capture of histidine-tagged proteins included.
An attractive approach for protein attachment
Capture of histidine-tagged recombinant proteins is an attractive approach for protein attachment in Biacore systems for a number of reasons, one being that physiological conditions can be used during the whole procedure. His capture kit is designed for capture of histidine-tagged proteins by an antihistidine antibody and provides an alternative to capture on nickel-chelated nitrilotriacetic (NTA) groups.
Lower non-specific binding and/or higher binding stability
Depending on the nature of the interacting molecules, this approach might give lower non-specific binding and/or higher binding stability. The anti-histidine antibody is a monoclonal antibody (MAb) directed against polyhistidine tags in the C or N terminus of a protein.
Product specifications
Order Information
The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1. Not for use with Biacore Flexchip
Kit contents
Anti-histidine antibody, immobilization buffer, and regeneration solution.
For use with
Enables antibody capture of histidine-tagged ligands in Biacore systems
Capacity
Sufficient for 10 immobilizations and 1000 injections.