Genetic mutation test kit Dx

for JAK2 mutationsbloodfor qPCR

Single-pot duplex assay with integrated reverse transcription (RT) / cDNA synthesis and qPCR. Minimal Residual Disease determination through BCR-ABL/ABL ratio reported on the International Scale (IS) when used in conjunction with the recommended GenePath Dx RNA extraction protocol SAMPLE TYPE: EDTA peripheral blood or bone marrow REACTION VOLUME: 15 μL LIMIT OF DETECTION: 0.0032% of BCR-ABL fusion transcript with ABL transcript used as the reference gene with a logarithmic reduction of 4.49 in relative expression between BCR-ABL and ABL (MR = 4.49). WORKFLOW: Uses the ‘UNG/dUTP’ system to minimize the risk of PCR carry-over contamination. The ABL transcript serves as a reference and internal control for monitoring the adequacy of sample and RNA extraction INSTRUMENT COMPATIBILITY: Bio-Rad CFX96, Bio-Rad Opus, QIAGEN Rotor-Gene Q, ABI QuantStudio 5, ABI 7500 and Roche Light Cycler 480 II RUN TIME: ~ 100 mins SOFTWARE: Secure online tool to assist with data analysis and calculate the BCR-ABL/ABL ratio as reported on the IS