• Products
  • Catalogs
  • News & Trends
  • Exhibitions

Reverse transcriptase reagent kit V5002
Taq DNA polymeraseRT-qPCR

reverse transcriptase reagent kit
reverse transcriptase reagent kit
reverse transcriptase reagent kit
reverse transcriptase reagent kit
reverse transcriptase reagent kit
reverse transcriptase reagent kit
reverse transcriptase reagent kit
Add to favorites
Compare this product
 

Characteristics

Type
Taq DNA polymerase, reverse transcriptase
Applications
RT-qPCR
Storage temperature

-20 °C
(-4 °F)

Description

The One-step Probe RT-qPCR Kit is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 2 × One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan®. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc. Applications • Probe gene expression analysis • Probe Low-copy gene detection • Probe microarray validation • Probe gene knockdown validation Features • One-step RT-qPCR • This kit is suitable for fluorescence quantification by probe method • This kit is compatible with many real-time systems • Hot-start technology brings high specificity and reproducible amplification • dUTP/UDG system, effectively prevent PCR product contamination
*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.