Reverse transcriptase reagent R3002

enzymeagarose gelnucleic acid

Gold Reverse Transcriptase is a new reverse transcriptase obtained by in vitro molecular evolution based on M-MLV (RNase H-) Reverse Transcriptase. The first strand of cDNA can be synthesized at 37~55°C. Gold Reverse Transcriptase has further significantly improved sensitivity, specificity, thermal stability and half-life, which is very suitable for reverse transcription of RNA templates with complex secondary structure. Gold Reverse Transcriptase has stronger polymerization and extension ability, which can be used for the synthesis of long cDNA and the construction of high proportion of full-length cDNA library. Unit Definition Poly (rA)·Oligo (dT) was used as the template/primer. At 37°C for 10 min, the amount of enzyme required to add 1 nmol dTTP as an acid-insoluble substance was defined as 1 unit of activity (U). Quality Control Exonuclease residue detection: 200 U of this product and 50 pmol single-stranded DNA substrate were incubated at 37°C for 16 h, and the DNA electrophoresis band did not change after denaturation PAGE electrophoresis. Detection of endonuclease residue: 200 U of this product and 0.3 μg of pBR322 DNA were incubated at 37°C for 4 h, and the electrophoresis bands of the plasmids were not changed by agarose gel electrophoresis. RNase residue detection: the product of 200 U and 1 μg of 293 cell RNA were incubated at 37°C for 30 min, and the electrophoresis band of RNA was unchanged by agarose gel electrophoresis. E.coli DNA residue detection: the nucleic acid residue in 60 U was detected by E. coli gDNA-specific TaqMan qPCR, and the residue of E. coli genome was less than 10 copies.