Microscope slide scanner MoxiePlex®

high-capacityautomaticbright field

Product overview
The MoxiePlex Multi-spectral imaging system captures high-resolution whole-slide images of tissue samples stained with multiple fluorescent dyes. Designed for spatial proteomics and translational research workflows, it provides 16-bit output and uses a high-quantum-efficiency CMOS camera covering approximately 400–900 nm to increase reagent compatibility and biomarker detection.

Key features

• Multiplex fluorescence imaging with up to 9 channels across an entire tissue section.
• 16-bit image output for high dynamic range and quantitative analysis.
• High-quantum-efficiency camera covering ~400–900 nm to broaden fluorescent reagent compatibility.
• Automated sample recognition, scan-range setting, focus-position determination, and exposure-time calculation to reduce manual setup and improve reproducibility.
• Unmixing technology to separate overlapping fluorescence signals and reduce autofluorescence components.
• Library creation function to generate unmixing libraries from single-stained images, including autofluorescence components for improved accuracy.
• Side-by-side visualization of brightfield and fluorescence images within the software (compatible with H&E and IHC imaging).
• Z-stack capability included for volumetric imaging.

Imaging capabilities
The system supports whole-slide fluorescence imaging at microscopy resolutions (20× and 40× modes). Supported output formats include OME-TIFF (16 bit and 8 bit) and NDPI (8 bit). Fluorescence acquisition can run with simultaneous unmixing to streamline acquisition-to-analysis workflows.

Automation & workflow

• Automated macro imaging for overview followed by automated sample detection to define scan ranges.
• Automatic exposure time calculation and optimal focus determination (patent pending) enabling fully automated operation or manual control as required.
• Concurrent unmixing processing during scanning to accelerate transition to analysis.

Compatibility and assays
The MoxiePlex supports a wide range of spatial biology assays and reagent panels from multiple vendors. It is compatible with brightfield modalities (H&E, IHC), providing morphological context alongside multiplex fluorescence data.

Notes
MoxiePlex is a registered trademark of Hamamatsu Photonics K.K. (China, EU, Japan, Switzerland, UK).

Technical specifications

• Product name: MoxiePlex Multi-spectral imaging system
• Product number: C16919-01
• Cassette loader: Up to 60 slides
• Compatible glass slide: 75.0 mm to 76.0 mm × 25.0 mm to 26.0 mm (Thickness: 0.9 mm to 1.2 mm)
• Objective lens: 20× NA 0.8
• Scanning resolution: 20× mode: 0.46 μm/pixel; 40× mode: 0.23 μm/pixel
• Brightfield scan speed: 20× mode: Approx. 60 s (15 mm × 15 mm); 40× mode: Approx. 70 s (15 mm × 15 mm)
• Fluorescence throughput*: 20× mode: Approx. 12 min (15 mm × 15 mm, 5 channels); 40× mode: Approx. 14 min (15 mm × 15 mm, 5 channels)
• Fluorescence camera: In-house CMOS camera
• Z-stack feature: Included
• Image formats: OME-TIFF (16 bit, 8 bit), NDPI (8 bit)
• Power supply: AC 100 V to AC 240 V
• Power consumption: Approx. 180 VA

*Fluorescence throughput time covers loading, macro photography, prefocusing, scanning, image processing and unloading.