Automatic nucleic acid analyzer T4

POClaboratoryveterinary

Introduction
Nucleic acid colorimetric detection reagent is an isothermal amplification (LAMP)-based assay formulated for visual color change readout. The amplified nucleic acid product interacts with a colorimetric dye to produce a clear color shift, enabling detection down to single-copy targets without complex instrumentation.

Product overview
The reagent delivers a visual readout: positive samples turn light yellow while negative samples remain bright rose red. It is available as a freeze-dried, room-temperature stable formulation that is re-soluble for testing. Designed to be used with a compact handheld visualization detector that maintains a constant reaction temperature (~65°C) and provides automatic color-signal interpretation within approximately 40 minutes.

Benefits

• Accurate – Single-copy target sensitivity reported; performance compared favorably to colloidal-gold methods.
• Portable – Compatible with a handheld detector (Rubik's-cube-sized) for field deployment.
• Simple – Pre-formulated, freeze-dried reagents and one-touch device operation.
• Rapid – Complete amplification and visual result within ~40 minutes.
• Connected – Detector supports Bluetooth for fast result retrieval and documentation.

Principle
Loop-mediated isothermal amplification (LAMP) combined with optical colorimetric detection. Amplification at a constant temperature (~65°C) induces a dye-dependent color change when the target sequence is present. Readout can be visual or performed by the matched handheld detector for automated interpretation.

Kits / Typical detection targets (examples listed on the product page)

• JDT001 SARS‑CoV‑2 Nucleic Acid Detection Kit
• JDT002 Monkeypox Nucleic Acid Detection Kit
• JDT003 Mycoplasma pneumoniae Nucleic Acid Detection Kit
• Examples for pets: Feline herpesvirus, Feline calicivirus, Mycoplasma felis, Chlamydia felis, Feline panleukopenia virus, Canine parvovirus, Canine distemper virus.
• Food and pathogenic organism kits (examples): Staphylococcus aureus, Escherichia coli O157:H7, Shigella spp., Listeria monocytogenes, Salmonella spp., Cronobacter (Enterobacter) sakazakii, Pseudomonas aeruginosa, Bacillus cereus, Vibrio parahaemolyticus.

Applications
Intended for rapid nucleic acid detection in medical and veterinary screening, pet infectious disease testing, animal husbandry, food safety monitoring, environmental hygiene checks, community or home self-testing and basic point-of-care screening. Suitable sample types include throat swabs, bronchoalveolar specimens and tissue exudates.

Advantages (detail)

• Fast: Amplification and readout completed within ~40 minutes.
• Accurate: Reported minimum detection limit ~1000 copies/mL for some kits (varies by target and kit).
• Portable: Handheld detector enables on-site testing without laboratory infrastructure.
• Intuitive: Clear colorimetric readout visible to the naked eye (light yellow = positive; bright rose red = negative).
• Easy to operate: Pre-assembled, freeze-dried reagents are re-soluble; matched detector enables one-touch testing.

Pet infectious disease — example table (excerpt)
Felinae | Feline herpesvirus | Signs: fever, cough, respiratory symptoms, viral bronchitis
Felinae | Feline calicivirus | Signs: oral inflammation, ulceration, respiratory symptoms
Felinae | Mycoplasma felis | Signs: conjunctivitis, respiratory symptoms
Felinae | Chlamydia felis | Signs: coughing, sneezing, conjunctivitis
Felinae | Feline panleukopenia virus | Signs: leukopenia, vomiting, hemorrhagic enteritis
Canis lupus | Canine parvovirus | Signs: leukopenia, vomiting, hemorrhagic enteritis
Canis lupus | Canine distemper virus | Signs: anorexia, depression, fever, high mortality risk

Notes
Visual positive = light yellow; visual negative = bright rose red. The matched handheld visualization detector performs amplification at a constant temperature (~65°C) and interprets the colorimetric signal automatically (typical judgment time within 40 minutes).

Technical specifications

• Product name: Nucleic acid colorimetric detection reagent
• Detection method: Isothermal amplification (LAMP) with colorimetric dye
• Result readout: Visual color change (light yellow = positive; bright rose red = negative)
• Time to result: Approximately 40 minutes
• Sensitivity: Minimum detection limit reported ~1000 copies/mL (varies by kit and target)
• Reagent format: Freeze-dried (room-temperature stable), re-soluble
• Device compatibility: Handheld visual detector operating at ~65°C; Bluetooth support for result checking
• Typical sample types: Throat swab, bronchoalveolar specimens, tissue exudate
• Typical target categories: Viral pathogens (e.g., SARS‑CoV‑2, feline/canine viruses), bacterial pathogens (foodborne and clinical)