Mycoplasma test kit

for infectious diseasesfor urinary diseasesfor sexually transmitted diseases

Product overview
The test kit consists of freeze-dried urea-arginine culture broth and susceptibility test boards. The broth contains peptone, yeast extract, serum, growth factors and nutrients, including urea and arginine. Alkaline products from urea/arginine metabolism raise broth pH and change color from yellow to red. The broth also contains antibacterial agents to inhibit bacterial and fungal contaminants. Test board wells are used for controls and for identification, enumeration and antibiotic susceptibility testing of Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH).

Application
For isolation, identification, enumeration and antibiotic susceptibility testing of urogenital Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH) in a single workflow.

Test principle
The kit uses freeze-dried urea-arginine broth and susceptibility test boards. Metabolism of urea and arginine produces alkaline compounds that change broth color from yellow to red, indicating growth. Selective antibacterial agents in the broth suppress bacteria and fungi. Test board wells provide control positions and specific wells for UU and MH identification/enumeration and for antibiotic susceptibility determination.

Main components

• Urea-arginine broth (freeze-dried): 20 vials
• Susceptibility test board: 20 pcs
• Sterile pipette tips: 20 pcs
• Sterile paraffin oil: 2 bottles
• Sterile diluting liquid: 2 bottles
• Instruction: 1 pc
• Report booklet: 1 pc

SUSCEPTIBILITY FOR TEST

• 10-antibiotic panel: Doxycycline (DOX), Minocycline (MIN), Ciprofloxacin (CPF), Ofloxacin (OFL), Sparfloxacin (SPA), Roxithromycin (ROX), Azithromycin (AZI), Clarithromycin (CLA), Josamycin (JOS), Spectinomycin (SPE)
• 12-antibiotic panel: Doxycycline (DOX), Minocycline (MIN), Ciprofloxacin (CPF), Ofloxacin (OFL), Sparfloxacin (SPA), Roxithromycin (ROX), Azithromycin (AZI), Clarithromycin (CLA), Josamycin (JOS), Spectinomycin (SPE), Acetylspiramycin (ASP), Levofloxacin (LEV)

Sample requirements

• Male: urethral swab/secretions, prostatic fluid, semen, or urine sediment (collect 10 ml urine, centrifuge 2000 rpm for 10 minutes)
• Female: cervical secretions or amniotic fluid collected appropriately
• Or: 30–50 μl freshly cultured positive broth

Results explanation

• Identification and enumeration: read and record Ureaplasma urealyticum (UU) results at 24 hours; read and record Mycoplasma hominis (MH) results at 48 hours.
• Antibiotic susceptibility: wells in line A contain lower antibiotic concentrations and line B higher concentrations; comparison of A and B for the same antibiotic determines susceptibility (kit materials include example layouts and interpretation guidance).

Additional product details (table data)
Product Name: MycoplasmaTest kit
Specimen: midstream urine or secretion
Method: pathological analysis equipment (in vitro diagnostic)
Form / Dosage Form: In Vitro Diagnostic rapid test cassette / freeze-dried format
Reading Time: rapid components reading within 10–15 minutes where applicable
Accuracy: >99.9%
Format: freeze-dried
Certification: CE, ISO13485, SFDA, FSC
Shelf Time: 24 months (table) / Storage and validity: store at 2–8°C; 12 months (text)
Package: 20 tests/box

Technical specifications

• Commercial name: MycoplasmaTest kit
• Brand: HWTAI
• Intended use: Isolation, identification, enumeration and antibiotic susceptibility testing of UU and MH (urogenital tract)
• Specimen types: midstream urine, urethral/cervical secretions, prostatic fluid, semen, urine sediment, amniotic fluid, or cultured positive broth
• Format: freeze-dried urea-arginine broth with susceptibility test boards
• Components per kit: 20 freeze-dried broth vials, 20 susceptibility boards, 20 pipette tips, 2 bottles paraffin oil, 2 bottles diluting liquid, 1 instruction, 1 report booklet
• Reading time: UU identification/enumeration at 24 h, MH at 48 h; certain rapid components readable within 10–15 minutes
• Accuracy: >99.9%
• Antibiotic panels: available in 10- and 12-antibiotic configurations (see main components)
• Storage: recommended 2–8 °C (text indicates 12 months under recommended storage; table indicates 24 months shelf life)
• Package size: 20 tests/box (20T/Kit)
• Certifications: CE, ISO13485, SFDA, FSC