Sample Size: 50-200µl whole human blood/up to 5×106cultured mammalian cells/1×109cultured bacterial cells
Expectant Yield: up to 5-30µg
Operation Time: 30min or less
Elution Volume: 50µl
Free sample available upon request
The Mini Total RNA Sample Kit for blood and cultured cells is specially designed for purification of total RNA from fresh whole human blood and cultured cells. This method uses detergents and a chaotropic salt to lyse cells and inactivate RNase. The RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed following the wash step, the purified RNA is eluted by RNase-Free water. The entire procedure can be completed within 30 minutes. The purified RNA is ready for RT-PCR, Northern Blotting, Primer Extension, and cDNA library construction.
The quality of the Total RNA mini Kit for blood and cultured cells is tested on a lot-to-lot basis by isolating total RNA from blood and cultured cell samples. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.
Specifications Mini Total RNA Kit (Blood & Cultured Cells)
Format: Spin Column
Binding Capacity: 50 µg
Sample Concentration: 300 µl of Whole blood 5 x 106Cultured animal cells
Sample Type: Blood and Cells
Typical Yield: 2-3 µg from 300 µl Blood 25-30 µg from 5 x 106Cells
Elution Volume: 50 µl
Operation Time: <20 mins.