Serum reagent kit IB47700
plasmafor DNA extractionfor RNA extraction

serum reagent kit
serum reagent kit
serum reagent kit
serum reagent kit
serum reagent kit
serum reagent kit
serum reagent kit
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Characteristics

Type
serum, plasma
Applications
for DNA extraction, for RNA extraction, tissue, blood sample, for nucleic acids, for PCR
Tested parameter
protein

Description

Sample Size: up to 5×106cultured animal cells/up to 25mg animal tissue /up to 500µl whole blood/up to 200µl biological fluids (serum, plasma) Expectant Yield: up to 9µg of genomic DNA/20µg of total RNA/120µg of protein from 1×106of HeLa cells Operation Time: DNA/RNA purification within 25 minutes, protein precipitation within 50 minutes Elution Volume: 50 – 200µl (genomic DNA)/25 – 50µl (total RNA) Format: genomic DNA spin column and total RNA spin column The DNA RNA Protein Extraction Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids. Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column. The flow-through can then be transferred to the RNA Spin column for RNA binding. Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water. DNA/RNA purification can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation, and protein purification can be completed in 50 minutes. The purified DNA, with approximately 20 – 30Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.

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