Solution reagent kit TruPath™ Genome

sample preparation

Overview
TruPath Genome is a whole-genome sequencing kit designed for comprehensive human WGS with minimal hands-on preparation. The workflow eliminates traditional library prep by performing tagmentation on the flow cell, delivering high-accuracy short reads augmented by mapped-read/proximity data for improved long-range genomic resolution.

Key features

• Minimal hands-on workflow: approximately 10 minutes of sample preparation.
• Combines high-accuracy short-read sequencing with mapped-read and proximity-enabled data for enhanced long-range information.
• Improved detection of structural variants and resolution in difficult-to-map regions, plus extended phasing capabilities.
• On-flow cell tagmentation removes the need for conventional library preparation prior to sequencing.
• Compatible with NovaSeq X Series systems.

Product literature and resources (selected)

• Data sheet, product flyers, application notes and technical notes detailing the TruPath Genome assay, sample performance and workflows.
• Support and software documentation covering TruPath Genome and DRAGEN germline analysis pipelines.
• Video demonstrations and product introduction materials explaining mapped-read technology and TruPath Genome workflow.

Specifications / technical details

• Assay time: ~29 hr (includes sequencing time).
• Description: Resolves difficult-to-map regions and provides comprehensive genome coverage with improved structural variant detection and phased reads/variant calls.
• Hands-on time: ~10 min.
• Input quantity: 350 ng.
• Instruments: NovaSeq X System; NovaSeq X Plus System.
• Mechanism of action: On-flow cell tagmentation.
• Method: Whole-genome sequencing.
• Nucleic acid type: DNA.
• Number of reactions: 2 or 8 samples per kit.
• Sample type details: High molecular weight DNA extraction recommended.
• Specialized sample types: Blood; Buccal swabs; Saliva.
• Species category: Human.
• Technology: Sequencing (mapped-read / proximity-enabled sequencing).
• Variant classes: Short tandem repeats (STRs); Single nucleotide polymorphisms (SNPs); Single nucleotide variants (SNVs); Insertions-deletions (indels); Copy number variants (CNVs); Structural variants; Gene rearrangements; Loss of heterozygosity (LOH); Chromosomal abnormalities; Germline variants.