DNA microarray BovineSNP50 v3

Overview
The BovineSNP50 v3 BeadChip is a 24-sample microarray for high-density bovine genotyping. It contains 53,218 informative SNP markers evenly distributed across the bovine genome to support genome-wide selection, QTL identification, evaluation of individual genetic merit and comparative genetic studies. The array was developed with USDA-ARS, University of Missouri and University of Alberta and integrates public bovine reference genome and Bovine HapMap data. Validated across 18 common beef and dairy breeds. Median marker spacing: ~37.4 kb.

Key features

• Fixed marker content: 53,218 evenly distributed SNPs across the bovine genome
• Custom marker add-on capacity: up to 600,000 additional markers with "+" kit versions
• 24-sample BeadChip format for medium-throughput genotyping
• PCR-free, single-tube sample preparation to reduce handling steps
• Validated across 18 common beef and dairy breeds; content derived from sequencing and public resources
• Designed for compatibility with LIMS and robotic automation for sample tracking and high-throughput workflows

Technical details
Assay type: Infinium HTS
Automation capability: Automated array loader; liquid handling robots supported
Input quantity: 200 ng DNA (recommended, at 50 ng/µL)
Compatible instrument: iScan System
Method: Genome-wide genotyping array (Infinium)
Nucleic acid type: DNA
Number of markers: 53,218 fixed SNP markers; custom add-on capacity up to 600,000 markers (with "+" kit versions)
Species category: Bovine (dairy and beef breeds)
Technology: Microarray
Median marker spacing: ~37.4 kb
Sample format: 24-sample BeadChip

Applications / Use cases

• Genome-wide association studies (GWAS) and QTL mapping in cattle
• Genomic selection and evaluation of breeding stock for dairy and beef programs
• Population genetics and comparative studies across breeds
• High-throughput, cost-effective genotyping for research and breeding programs

Technical specifications

• Assay type: Infinium HTS
• Automation capability: Automated array loader; supports liquid handling robots
• Input DNA: 200 ng (recommended, at 50 ng/µL)
• Compatible instrument: iScan System
• Method: Genome-wide genotyping array (Infinium)
• Nucleic acid type: DNA
• Number of markers: 53,218 fixed SNPs; custom add-on capacity up to 600,000 (with "+" kit versions)
• Species: Bovine (dairy and beef breeds)
• Median marker spacing: ~37.4 kb
• Sample format: 24-sample BeadChip