The UltraSYBR Mixture (Low ROX) is a premixed system for real-time fluorescence quantitative PCR (SYBR Green I), and the concentration is 2×. It contains GoldStar Taq DNA Polymerase, PCR Buffer, dNTPs, SYBR Green I Fluorescent Dye, Mg2+ and Low ROX as reference dye. The operation is simple and convenient. This product is mainly used for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription.
This product contains the fluorescent dye SYBR Green I which can bind with all double-strand DNA, so that the product can be used for the detection of different target sequences without the need for the synthesis of specific labeled probes. The GoldStar Taq DNA Polymerase in the mixture is a chemically-modified, new efficient hot-start enzyme that does not have polymerase activity at room temperature which prevents non-specific amplification efficiently, and it is activated by incubation at 95°C for 10 minutes. The combination of a unique PCR buffer system and a hot-start enzyme effectively inhibits non-specific PCR amplification and significantly increases the amplification efficiency of PCR.
The ROX dye contained can correct the fluorescence signal error between the wells of the quantitative PCR instrument. The amount of ROX in this kit is low, and it is suitable for quantitative PCR instruments which require low ROX for signal correction, such as ABI Prism 7500/7500 Fast, Stratagene Mx3000/MX3005P, and Corbett Rotor Gene 3000.