Enzyme reagent kit

DNA polymeraseNGSfor DNA library preparation

This kit is specially developed for the Illumina high-throughput sequencing platform. It provides the enzyme premix system and reaction buffer required for genomic DNA library construction, including all components except PCR primers. Compared with traditional library construction kits, this kit uses a novel transposase method for library construction, which can complete DNA fragmentation, end repair and adapter ligation in one simple enzymatic reaction, significantly reducing the amount of template used. , reducing the experimental operation steps and shortening the library construction time; using high-fidelity DNA polymerase for library enrichment, unbiased PCR amplification, expanding the coverage area of the sequence, and efficiently preparing DNA for the Illumina next-generation sequencing platform library. This kit is suitable for starting template DNA input of 50 ng. All reagents in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.