Research assay kit LS-K297

for drug discoveryfor ASTNAD

Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and a-ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver. AST is elevated in liver and muscle diseases. It is part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease and trauma. Simple, direct and automation-ready procedures for measuring AST activity find wide applications in research and drug discovery. This AST activity assay is based on the quantification of oxaloacetate produced by AST. In this assay, oxaloacetate and NADH are converted to malate and NAD by the enzyme malate dehydrogenase. The decrease in NADH absorbance at 340 nm is proportionate to AST activity. Specifications Type Detection/Quantition Usage For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity. Target Aspartate Transaminase Detection Colorimetric (340 nm Absorbance) Supplied Components The following components are supplied with this product. Assay Buffer Cofactor Enzyme Mix NADH Reagent: Dried (See Datasheet for specific volumes supplied) Applications Spectrophotometry (visible) Equipment Microplate spectrophotometer (visible), Spectrophotometer (visible) Conditions Shipped -20°C Dry Ice, Store at -20°C, 6 months shelf life.