Solution reagent LS-M36

for ELISA

TMB Substrate (Super Sensitive) is a ready-to-use TMB substrate for microwell applications containing 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer. This substrate is our most sensitive TMB buffer. This sensitive, faster kinetic TMB substrate is approximately 40-fold more sensitive than our most popular substrate, TMB Substrate (ELISA). Specifications 3,3',5,5'-tetramethylbenzidine (TMB) substrates react with peroxidase, yielding a soluble blue reaction product. When the TMB-peroxidase reaction is stopped using Stop Solution for (TMB), the chromogen changes from blue to yellow, and the absorbance changes from 620 nm to 450 nm. This product is ideal for detecting very low levels of a target molecule, running samples that must be highly diluted (1:10,000), amplifying the signal when using antibodies with low binding capacity, running samples that exhibit high steric hindrance, and shortening the incubation time of the assay. • Contains: No proteins • Supplied at: 1X