Salmonella reagent xMAP®

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Salmonellosis is the most frequently reported cause of foodborne illness, causing an estimated 1.4 million cases of foodborne illness and over 400 deaths annually in the US.1 The Luminex xMAP® Salmonella Serotyping Assay (SSA) is a multiplex, nucleic acid-based assay for research or epidemiological use in identifying serotypes greater than 85%2 of the most commonly encountered Salmonella isolates within hours compared with existing methods that can take up to six weeks for results. The xMAP Salmonella Serotyping Assay was developed by the CDC3-4 for fast and comprehensive molecular Salmonella serotyping. The assay consists of three separate tests that detect O and H antigens simultaneously, including the ability to identify some serotype-specific markers in the Additional Targets or AT test. Tests for 7-O Groups, 35-H antigens & three additional targets Built on proven Luminex technology: MicroPlex® Microspheres and Luminex® 100/200™ Each kit has reagents for 100 samples and 20 uses Workflow Step 1 DNA is extracted and quantified from a Salmonella isolate using a simple 15 minute extraction method. Step 2 Multiplexed PCR with biotinylated primers is performed for each of the three tests included in the assay. Step 3 Labeled amplicon is hybridized to the appropriate oligonucleotide probe-coupled microsphere mixture and labeled with SAPE reporter. Step 4 The assay plate is analyzed on a Luminex® 100/200™. Antigen Targets cCovered by xMAP® SSA Test Groups/Antigens Detected O-Group - B, C1, C2, D, E, G, and serotype Paratyphi A (ParaA)