DNA ladder reagent / for DNA quantification / for PCR / for enzymes
AccuRuler 100bp Plus
for DNA quantification, for PCR
AccuRuler 100bp Plus DNA Ladder
Description Size: 50pg / 500pl
The AccuRuler 100 bp Plus DNA RTU Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 3,000 bp.
The 500 bp and 1,500 bp bands have increased intensity to provide internal orientation. The mass of DNA in each band is provided (assuming a 0.5 pg/ 5 pi load) for approximate quantification in comparably intense samples of similar size.
Source PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCI (pH 8.0) andlmM EDTA.
Range: 100 bp to 3,000 bp Number of bands: 12 Concentration: 100 pg/ml Recommended load: 5 pi per well
Storage and loading buffer: 10 mM Tris-HCI (pH 8), 10 mM EDTA, orange G, xylene cyanol FF and glycerol.
Storage: Store at -20°C for 12 months
Accurate size and mass estimations
DNA Ladders comprising of fragments of specific base pair length are available for estimation of molecular weights from lOObp to 10Kb. For easy determination of band size, several DNA Ladders include bands that are two to three times more intense.