Electrophoretic light scattering particle size analyzer Zetasizer Pro

opticaldynamic light scatteringlaser light scattering

Overview
The Zetasizer Pro is a laboratory analyzer for particle and molecular size, electrophoretic mobility, zeta potential and molecular weight measurement. It uses Non‑Invasive Back Scatter (NIBS) optics and Adaptive Correlation to provide high sensitivity, wide dynamic concentration capability and reduced measurement time. A deep learning–based data quality system evaluates results and gives guidance to improve measurements.

Key capabilities

• Measure particle size, molecular size, electrophoretic mobility, zeta potential and molecular weight
• NIBS optics for high sensitivity and broad concentration range
• Adaptive Correlation to shorten measurement time and improve reproducibility
• Deep learning data quality assessment with actionable guidance
• Optical filter wheel (fluorescence and polarization filters) for fluorescent samples and DDLS
• ZS Xplorer software for method setup and data analysis

Product variants
Available as Blue Label or Red Label:

• Blue Label: for routine samples (e.g. metallic colloids, metal oxides, pigments)
• Red Label: for challenging samples (e.g. proteins, surfactant solutions, low solid content)

Features (detailed)

• Dynamic Light Scattering (DLS): size range 0.3 nm to 10 µm
• M3‑PALS with Constant Current Mode for zeta and electrophoretic mobility
• Electrophoretic Light Scattering (ELS) for zeta-based stability assessment
• NIBS optics for versatile, high‑sensitivity sizing and molecular weight (Static Light Scattering)
• Optical filter wheel with fluorescence and polarization filters supporting DDLS
• Range of disposable and reusable cells including low‑volume and capillary options
• On‑site upgrade path to Zetasizer Ultra and optional MPT‑3 Autotitrator

Which Zetasizer is right for me? (comparison highlights)

• Size measurement: Pro — NIBS + DLS (0.3 nm–10 µm)
• Optical filter wheel (Fluorescence/DDLS): Pro — Yes
• Minimum volume (size): Pro — 12 µL
• Molecular weight capability: Pro — Static Light Scattering (173°)

Key applications

• Colloidal silica: reproducible sizing for QC
• Titanium dioxide (TiO2): stability and formulation studies (with MPT‑3 for pH/salt titration)
• Ceramics: population identification using MADLS where applicable
• Proteins: early‑stage characterization with low sample volumes
• Fluorescent nanoparticles (carbon dots): measurement using filter wheel to remove interference
• AAV and viral samples: size distribution and concentration workflows (MADLS on Ultra for titering)

Industry applications

• Academic research and development
• Bioscience and biopharmaceutical formulation development
• Food & beverage for dispersion and stability analysis
• Nanomaterials characterization
• Paints, inks & coatings formulation control
• Pharmaceuticals: dispersion and emulsion quality control

Documents and software
Downloadable product brochure, user manuals and software updates available on the product page. ZS Xplorer and optional OmniTrust support regulated workflows.

Technical specifications

• Measurement principles: NIBS, DLS
• Measurement angles: 173° and 13°
• Size range: 0.3 nm–10 µm
• Minimum sample volume (size): 12 µL; (zeta): 20 µL
• Concentration range (size): Blue Label ~0.2 mg/mL (15 kDa protein) to 40% w/v; Red Label ~0.1 mg/mL to 40% w/v
• Zeta range: 3.8 nm–100 µm; conductivity up to ~260 mS/cm
• Static Light Scattering (molecular weight): 300 Da–20 MDa (±10%)
• Detector: Avalanche Photodiode; Laser: He‑Ne 633 nm (Blue 4 mW max; Red 10 mW max)
• Temperature control: 0 °C–120 °C (dry N2 purge below dewpoint)
• Dimensions (W×D×H): 322 × 565 × 245 mm; Weight: 19 kg
• Power: AC 100–240 V, 50–60 Hz; Typical 45 W, Max 100 W
• Interface: USB 2.0 or higher; Standards: ISO22412:2025, ISO13099‑2:2025, relevant USP & ASTM methods
• Accessories: disposable/reusable cells, capillary cells, high‑concentration zeta kit, MPT‑3 Autotitrator, sample assistant