Research assay kit Fura-2 QBT

calciumcell

For researchers concerned about assay artifacts, the Fura-2 QBT™ Calcium Kit is a simple, mix-and-read format that employs our proprietary masking technology with the industry-standard Fura-2 ratiometric calcium indicator to accurately measure calcium mobilization. The Fura-2 QBT Calcium Kit eliminates the cause of data variability and reduces the number of steps compared to conventional wash protocols using Fura-2. Leveraging our proprietary masking technology with the industry standard Fura-2 ratiometric calcium indicator, researchers can: See largest Fura-2 signal window available Eliminate wash artifacts and increase throughput with homogeneous assay Minimize the impact of uneven dye loading and leakage on results Interrogate low-density, weakly or non-adherent cells using no-wash protocol Assay on FlexStation® 3 and FLIPR® Tetra Systems Reference thousands of citations where Fura-2 has been used for cell lines and target Agonism of the Muscarinic M1 Receptor on CHO M1 Cells On the FlexStation® 3 Multi-Mode Microplate Reader, carbachol was used to stimulate the muscarinic M1 receptor on CHO M1 cells to generate concentration response curves (CRC), comparing Fura-2 QBT Dye to the BD Kit and the traditional Fura-2 wash protocol. EC50 values were similar for all methods, and were within range of published values. Antagonism of the Muscarinic M1 Receptor on CHO M1 Cells On the FlexStation 3 Multi-Mode Microplate Reader, 50 nM carbachol was used as agonist challenge versus an antagonist CRC of atropine. This experiment Fura-2 QBT Calcium Kit provided the largest signal window and most robust Z-factors at EC80.