The ezPatch 100A is a smart manipulator. It has all the functions a manual manipulator has. In addition, it comes with a computer controlled feedback system so that it can stop precisely after the electrode touches the cell membrane. The system is ideal for patch clamping.
In patch clamp experiments, all users need to do is to aim the electrode at a selected cell. A click of a button and the electrode moving at a specified speed will stop at the surface of the cell membrane at specified pressure (measured by resistance increase). If used with our ez-gSEAL pressure controller, suction will be automatically appled to make seal and suction pulses will be applied for going whole cell. Patch clamp experiments will be consistent and precise once you find the best seal parameters. The seal parameters can be saved for different cell types and shared among lab members.
The control software has many functions that make patch clamp experiments easy and precise. You can excise patches at a predefined speed. With the manual control functions
in the software, you can move the electrode forwards or backwards in as small as 0.1 µm steps at a defined speed. The software can also control multiple manipulators. Therefore, users can make seals on the same cell or a few different cells simultaneously.
ezPatch 100A If drift from the pipette holder/o-ring is a concern, an optional pipette-holder clamp can be purchased. It clamps the glass pipette so that the drift caused by an o-ring is minimal.