Polyacrylamide gel electrophoresis system FastGene®

SDSfor proteinsbenchtop

Complete set for PAGE protein analysis Most flexible use for 1−4 (pre-cast or hand-cast) gels Compatible with different pre-cast gels (Bio-Rad™, ThermoFisher, FastGene®) Hand-cast gel set included (1 mm gels) Easiest gel hand-casting − No leaking! Glass plate holder and tube holder included Additional 0.75 mm / 1.5 mm hand-cast sets available Details Powerful protein analysis via PAGE PAGE stands for Polyacrylamide Gel Electrophoresis and describes an analytical method in biochemistry for the separation of differently sized protein mixtures in an electric field. During PAGE, proteins migrate through a gel matrix in response to an applied electic field. Smaller proteins travel faster through the gel than larger proteins, leading to a size dependent separation. The most popular form of PAGE is SDS-PAGE. The detergent sodium dodecyl sulfate (SDS) is added for PAGE sample preparation and is also part of the buffer composition. The treatment with the harsh detergent leads to full protein denaturation and unfolding. SDS binds to hydrophobic parts of the unfolded protein and masks the intrinsic charge of the protein with its own negative charge. As a consequence, SDS-protein complexes migration is predominantly dependent on the size of the protein, allowing an estimation of its molecular weight.