Enzyme reagent kit VeriFi™

DNA polymerasedyeTaq DNA polymerase

Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. VeriFi™ Polymerase was formerly known as PCRBIO VeriFi™ Polymerase Features High temperature cycling – up to 100 °C denaturation to better separate GC-rich templates Increased PCR success rates with complex genomic templates (17.5 kb and over) High yields under standard and fast PCR conditions (10-30 s/kb) Efficient and specific amplification from challenging templates including GC and AT-rich sequences 100x higher fidelity than Taq DNA polymerase Generates blunt-end PCR products Available as a convenient 2x ready mix with the option of a red dye for direct gel loading More Information VeriFi™ Polymerase is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. The enzyme is engineered with proprietary mutations that significantly increase processivity, resulting in shorter extension times (10-30 s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5 kb. The high accuracy and enhanced 3’-5’ exonuclease activity of VeriFi™ Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended.