DNA polymerase reagent kit IsoFast™

dyefor isothermal amplificationhot start

st DNA polymerase combined with AptaLock™ reversible hot-start technology power our IsoFast™ Hot Start Bst reagents. These products are ideal for all isothermal amplification workflows, giving more sensitive and reliable results faster than ever before. IsoFast™ Hot Start Bst Polymerase is a recombinant version of the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus, Bst) DNA Polymerase. It is engineered to retain 5’-3’ polymerase activity, but lacks 5’-3’ exonuclease activity. The enzyme’s strong strand displacement capability eliminates the need for denaturation required by standard PCR. Combined with powerful hot start technology, to minimise primer dimer formation and non-specific target amplification, this novel formulation is ideal for non-PCR based amplification. Features: AptaLock™ hot start for ultra-sensitive detection of DNA targets Rapid polymerisation for faster time to results (as little as 10 mins) Detect down to 3 target copies per μL Ideal for both cold and room temperature setup Improved speed and sensitivity for early target detection High activity at a broad range of temperatures from 55-70 Available with and without fluorescent dye, and as a 2x ready mix Formats available for colourimetric assays. More Information IsoFast™ Hot Start Bst Polymerase & Mix are the most recent and improved development of isothermal amplification technology, designed to deliver unparalleled specificity and speed in DNA amplification processes and designed to meet the demands of molecular diagnostics and research.