The iTRAQ Reagents are the first set of multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples enabling simultaneous identification and quantitation, both relative and absolute, while retaining important PTM information. There are two types of iTRAQ reagent kits, 4plex and 8plex. The iTRAQ Reagents - 8plex kits are also available in more economical bulk packs.
Simultaneously identify and quantify proteins from multiple samples
Expands protein and proteome coverage by labeling all peptides, including those with post-translational modifications (PTMs)
Increases confidence in identification and quantitation from MS/MS spectra by tagging multiple peptides per protein
Increases throughput and confidence in results for protein biomarker discovery studies
Offers a simple workflow without sample fractionation for reduced-complexity samples, such as affinity pull-downs
Provides the flexibility to multiplex up to eight different biological samples simultaneously in a single experiment
Fully supported by ProteinPilot™ Software on all SCIEX proteomics LC/MS/MS platforms
Innovation in peptide-tagging chemistry
iTRAQ® Reagents are an isobaric, peptide-tagging technology that enable you to label all primary amines, regardless of peptide class. Because important information, such as post-translational modifications (PTMs), is retained, this tagging system allows you to extract more detailed information from your samples.
Higher-throughput protein biomarker discovery