The mTRAQ Reagents are amine-specific, stable-isotope-labeled reagents, available in duplex or triplex format, designed for high-confidence protein and peptide biomarker verification using multiple reaction monitoring (MRM) analysis on QTRAP Systems and the Triple Quad LC/MS/MS Systems.
The use of this triplex chemistry will increase sample throughput by 100%.
Duplex or triplex mass-difference tags (non-isobaric amine tags) available
Established chemistry with >95% labeling efficiency
High-selectivity, MRM-based, relative quantitation of proteins/peptides/PTMs
Global Internal Standard (GIS) workflow is supported for robust, reproducible, quantitative MRM assays in biomarker verification
Fractionation-compatible reagent
Easy transition from protein discovery based on labeling with iTRAQ Reagents to verification
Significantly more economical approach compared to use of stable-isotope-labeled synthetic peptides for biomarker verification when moitoring larger numbers of peptides
Biomarker verification supported by a complete suite of software, including ProteinPilot, MRMPilot, and MultiQuant Software
Innovative peptide-tagging chemistry: The mTRAQ® Reagents are non-isobaric, peptide-labeling reagents that enable you to label all primary amines, regardless of peptide class. The mTRAQ® Reagents are an extension of the iTRAQ® Reagent technology and are chemically identical, aside from isotope content (Figure 1).