Signal enhancement maximized through intelligent chemistry
Amplifex Diene reagent is designed specifically to enhance signal strength up to 1000-fold (depending on the analyte) and can be used to specifically derivatize any molecule with a cis-diene system, such as vitamin D3, vitamin D2 and their analogs
With the Amplifex Diene reagent, you can drastically reduce sample size and achieve ultra-low LLOD and LLOQ levels that were difficult, if not impossible, to achieve by mass spectrometry even on the most-sensitive platforms.
Intelligent design boosts ionization efficiency and improves fragmentation
Substantial reduction in sample volume and analysis time
Reactions are simple (one-step derivatization) and fast (30 minutes)
Generic derivatization technique can be used with any molecule that has a cis-diene system, such as a broader range of vitamin D3 and vitamin D2 analogs that are natural or synthetic
Now provided as a solid reagent for an increase of over 30% in shelf life compared to the previous liquid formulation
Amplifex Diene reagent is a simple, quick chemistry that improves ionization and enhances sensitivity for any cis-diene system, enabling workflows that push the limits of sample volume.
Signal enhancement for molecules with cis-diene functionality
1,25-dihydroxy vitamin D3 derivatized withAmplifex Diene reagent compared to underivatized provides a ~165-fold signal enhancement.
Signal enhancement for other molecules with cis-diene functionality that have been derivatized with Amplifex Diene reagent.