The oxygen partial pressure at which hemoglobin saturation is 50% is referred to as P50. This is a commonly used indicator of the affinity between hemoglobin and oxygen, as well as the oxygen release function of hemoglobin. The Hemox Analyzer is a device used to record the oxygen balance curve in blood (the process of hemoglobin binding to and releasing oxygen).
Based on the different light absorption characteristics of deoxygenated hemoglobin (Hb) and oxygenated hemoglobin (HbO₂), the system utilizes a dual-wavelength spectrophotometric method to detect the light absorption values of hemoglobin. After applying calculation formulas, these values are plotted as the Y-axis. A fluorescence quenching oxygen sensor is used to measure the absolute oxygen partial pressure in the sample (unit: mmHg), plotted on the X-axis to generate the oxygen binding and oxygen dissociation curve.
The Oxygen Binding Capacity Testing System can be used to test dissolved oxygen in blood, red blood cell lysates, hemoglobin products, etc. It also provides software analysis functions to generate hemoglobin-oxygen binding and dissociation curves and automatically analyze the P50 and Hill coefficient.
Product Features
Fully Automatic Testing
Professional Control and Analysis Software
Data Analysis:
Oxygen saturation curve
Oxygen dissociation curve
Oxygen saturation
Oxygen partial pressure
P50
Hill coefficient
Assay Principle
The changes in absorption values reflect the oxygen saturation level. A fluorescence quenching oxygen sensor measures the absolute oxygen partial pressure in the sample, and the software automatically generates the oxygen binding and dissociation curve.